The Role of Postbiotic Composition in the Growth Stimulating of Bifidobacteria

Introduction: Probiotic microorganisms are known to increase the biological value of foods, reduce cholesterol levels, positively affect the immune system, prevent intestinal infections and diarrhea associated with antibiotics, reduce symptoms of lactose intolerance, etc. These positive effects depend on the properties of the probiotic strain. The metabolic products of probiotic microorganisms are also capable of having a positive effect on the human body. The metabolic complexes secreted by probiotic bacteria are characterized by high digestibility and resistance to environmental conditions and can potentially be used along with probiotic microorganisms.

Purpose: To study. the possible effect of different concentrations of the postbiotic composition on enhancing the biological properties of the product, in particular, its ability to stimulate the growth of bifidobacteria.

Materials and Methods: As objects of research, a fermented milk product based on a probiotic association consisting of Lactococcus cremoris 241C, Lactocaseibacillus rhamnosus F, Propionibacterium shermanii E2, developed using a postbiotic complex (PC) in concentrations of 0.5 and 0.01%, was used. In the study of the ability to stimulate the growth of bifidobacteria, a strain of Bifidobacterium adolescentis MS–42 from the collection of FGANU «VNIMI» was used as a control culture. The studies were carried out on the GMC 2 medium and a probiotic fermented milk product developed on sterile skimmed milk. The effect of two PC concentrations (0.5 and 0.01%) on stimulating the growth of bifidobacteria in experimental samples after 8 and 24 hours of incubation was studied.

Results: The positive effect of PC at a concentration of 0.01% on the growth of bifidobacteria was confirmed. The absence of a stimulating effect of PC in a concentration of 0.5% may be due to inhibition by acetic acid - the final product

Conclusion: The data obtained allow us to confirm the increase in the biological effectiveness of a fermented milk product with a postbiotic complex in relation to stimulating the growth of bifidobacteria, and recommend it as an additive to the biotechnological system at a concentration of 0.01%.

1. Havkin A i dr. (2021). Kislomolochnye probioticheskie produkty - pishcha ili lekarstvo? Voprosy detskoj dietologii, T. 19, №. 3, 58-69

2. Islam S. U. (2016). Clinical uses of probiotics. Medicine, T. 95, №. 5,1-5

3. Saulnier D. M. et al. (2011). Exploring metabolic pathway reconstruction and genome-wide expression profiling in Lactobacillus reuteri to define functional probiotic features. PloS one, T. 6, №. 4, 183-187

4. Zobkova Z.S., Kondratenko V.V., Pryanichnikova N.S., Zenina D.V., Korovina N.S. (2023). Razrabotka i adaptaciya prognosticheskoj modeli polnofaktornoj optimizacii tekhnologicheskogo processa proizvodstva probioticheskogo kislomolochnogo produkta. FOOD METAENGINEERING.1(4), 48-56 https://doi.org/10.37442/fme.2023.4.30

5. Agarkova E.YU., Kruchinin A.G., Ryazanceva K.A. (2016). Sovremennye tekhnologicheskie podhody k obogashcheniyu molochnyh produktov. Innovacionnye tekhnologii obogashcheniya molochnoj produkcii (teoriya i praktika), 110-142

6. Donskaya G.A., Drozhzhin V.M. (2020). Biologicheski aktivnye ingredienty v kislomolochnyh produktah. Pererabotka moloka, №. 7, 20-23

7. Shigwedha N. et al. (2014). Probiotical cell fragments (PCFs) as “novel nutraceutical ingredients”. Journal of Biosciences and Medicines, T. 2, №. 03, 43

8. Tomar S. K. et al. (2015). Role of probiotics, prebiotics, synbiotics and postbiotics in inhibition of pathogens. The Battle against Microbial Pathogens: Basic Science, Technological Advances and Educational Programs; Méndez-Vilas, A., Ed, 717-732

9. Begunova A.V., ZHizhin N.A. (2022). Ocenka potenciala propionovokislyh bakterij dlya polucheniya postbiotikov. Hranenie i pererabotka sel'hozsyr'ya, 4, 105-112. https://doi.org/10.36107/spfp.2022.356

10. LeBlanc J. G. et al. (2013). Bacteria as vitamin suppliers to their host: a gut microbiota perspective. Current opinion in biotechnology, T. 24, №. 2, 160-168. DOI:10.1016/j.copbio.2012.08.005

11. Kapustian A. I. et al. (2018). Products of metabolism and processing of lactic acid bacteria as functional ingredients. Food Science and Applied Biotechnology, T. 1., №. 1, 47-55.

12. Begunova A.V., ZHizhin N.A. (2023). Harakteristika metabolitnogo kompleksa, produciruemogo L.reuteri LR1. FOOD METAENGINEERING, 1(3), 11- 20. https://doi.org/10.37442/fme.2023.3.28