foodmetaFOOD METAENGINEERINGFOOD METAENGINEERING2949-6497All-Russian Dairy Research Institute10.37442/fme.2023.3.24foodmeta-24Research ArticleОбзорная статьяScoping ReviewФерментативный способ производства биоактивных пептидов из молочного белкового сырья: обзор предметного поляEnzymatic Method of Bioactive Peptides Production from Milk Protein Raw Materials: A Scoping Reviewhttps://orcid.org/0000-0002-3227-8133КручининАлександр ГеннадьевичKruchininAleksandr Gennadievicha_kruchinin@vnimi.orghttps://orcid.org/0000-0002-8427-0387БольшаковаЕкатерина ИвановнаBolshakovaEkaterina Ivanovnae_bolshakova@vnimi.orgВсероссийский научно-исследовательский институт молочной промышленности (ФГАНУ «ВНИМИ»)РоссияAll-Russian Dairy Research InstituteRussian Federation202315122023134765Copyright © Кручинин А.Г., Большакова Е.И., 20232023Кручинин А.Г., Большакова Е.И.Kruchinin A.G., Bolshakova E.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.fme-journal.org/jour/article/view/24Введение

Введение: На протяжении 14 лет интерес к использованию молочного сырья для производства биологически активных пептидов (БАП) с антигипертензивным, антиоксидантным и антидиабетическим действием находился в фазе стремительного роста по причине необходимости профилактики различных заболеваний. На сегодняшний день особое внимание уделяется ферментативному способу производства, так как он позволяет направленно осуществлять процесс высвобождения БАП с заданными свойствами.

Целью данного обзора предметного поля являлось обобщение и систематизация опыта международных исследований за последние 14 лет в области ферментативного гидролиза как способа высвобождения БАП из молочного сырья.

Материалы и методы

Материалы и методы: Данный обзор предметного поля был выполнен в соответствии с руководством PRISMA-ScR. Поиск научных трудов был осуществлен в базе данных Google Scholar, так как она позволяет осуществлять поиск научных источников без ограничений по их формату и виду представления научных данных (книга, диссертация, статья), а также включает в себя научные источники из других баз данных и производит сортировку по релевантности.

Результаты

Результаты:  При поиске было выявлено 72 источника, опубликованных с 2007 по 2021 гг. на английском языке. Отобранные работы посвящены вопросам гидролиза микробными протеазами (МП) – 50%, и коммерческими ферментными препаратами (КФП) – 50%.

Выводы

Выводы: Существует ряд задач, решение которых необходимо для повышения уровня коммерциализации научных данных о процессе производства БАП посредством направленного гидролиза. Создание новых баз данных; наращивание исследовательской базы по совместной ферментации микроорганизмами и коммерческими ферментами; определение генов протеаз методом ПЦР позволят повысить практическую применимость исследований и обоснованность проведения бóльшего количества доказательных in vivo и клинических исследований.

Introduction

Introduction: For 14 years, interest in the use of dairy raw materials for the production of bioactive peptides (BPs) with antihypertensive, antioxidant and antidiabetic effects has been in a phase of rapid growth because of the need to prevent different diseases. Particular attention is being paid to enzymatic production method, as it allows for a targeted process of BP extraction with specified properties.

The  purpose of this scoping review was to summarize and systematize the experience of international research over the last 14 years in the field of FG as a method to extract BPs from milk protein substrates.

Materials and Methods

Materials and Methods: This scoping review was performed in accordance with the PRISMA-ScR guidelines. Scientific papers were searched in the Google Scholar database, because allows searching for scientific sources without restrictions on their format and type of scientific data presentation (book, dissertation, article), as well as includes scientific sources from other databases and performs relevance sorting.

Results

Results:  The search identified 72 sources published between 2007 and 2021 in English. Included sources are devoted to hydrolysis by starter bacteria enzymes (SBE) - 50%  and by commercial enzyme preparations (CEP) - 50%.

Conclusion

Conclusion: There are a number of challenges that need to be addressed in order to increase the level of commercialization of BP production through directed hydrolysis. Creation of databases; building up the research base on co-fermentation by microorganisms and commercial enzymes; conducting PCR studies of microbial protease genes will increase the practical applicability of research and the validity of conducting more evidence-based in vivo and clinical studies.

 

 

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The authors declare that there are no conflicts of interest present.